Ntle le likotsi tse tloaelehileng tsa polokeho libakeng tse ngata tsa mosebetsi tsa letsatsi le letsatsi (joalo ka likotsi tsa motlakase le mollo), lilaboratori tsa setso sa lisele li boetse li na le likotsi le likotsi tse ngata tse amanang le ho sebetsana le ho qhekella ha lisele tsa batho kapa liphoofolo le lisele, le chefo, e senyang kapa e fetohang. solvents.Li-reagents.Likotsi tse tloaelehileng ke ho phunngoa ka phoso ha linale kapa linoko tse ling tse silafetseng, ho qhalana le ho fafatsa letlalong le lera la mamina, ho kenngoa ka lipeipi tsa molomo le ho hema lierosoli tse tšoaetsanoang.
Sepheo sa mantlha sa lenaneo lefe kapa lefe la ts'ireletso ea likokoana-hloko ke ho fokotsa kapa ho felisa ho pepeseha ha basebetsi ba laboratori le tikoloho ea kantle ho li-agent tse ka bang kotsi tsa likokoana-hloko.Ntho ea bohlokoa ka ho fetisisa ea polokeho lilaboratoring tsa setso sa lisele ke ho latela ka tieo mekhoa le mekhoa e tloaelehileng ea microbiological.
1. Boemo ba Biosafety
Melao le likhothaletso tsa US mabapi le polokeho ea bophelo bo botle li teng tokomaneng ea "Biosafety in Microbiology and Biomedical Laboratories" e lokiselitsoeng ke Centers for Disease Control (CDC) le National Institutes of Health (NIH) mme e phatlalalitsoe ke ts'ebeletso ea Lefapha la Bophelo la US.Tokomane ena e hlalosa maemo a mane a nyolohang a tšireletso, a bitsoang maemo a 1 ho isa ho a 4 a biosafety, 'me e hlalosa mekhoa ea likokoana-hloko, lisebelisoa tsa polokeho, le mehato ea ts'ireletso ea lits'ebeletso bakeng sa maemo a kotsi a amanang le ho sebetsana le likokoana-hloko tse itseng.
1.1 Biosafety Level 1 (BSL-1)
BSL-1 ke tekanyo ea motheo ea tšireletso e tloaelehileng lilaboratoring tse ngata tsa lipatlisiso le tsa bongaka, 'me e loketse li-reagents tseo ho tsejoang hore ha li bake maloetse ho batho ba tloaelehileng le ba phelang hantle.
1.2 Biosafety Level 2 (BSL-2)
BSL-2 e loketse litlhare tse kotsi tse mahareng tse tsejoang hore li baka mafu a batho a matla a fapaneng ka ho ja kapa ka ho pepeseha ha transdermal kapa mucosal.Boholo ba lilaboratori tsa setso sa lisele li lokela ho fihlela bonyane BSL-2, empa litlhoko tse ikhethileng li ipapisitse le mohala oa sele o sebelisitsoeng le mofuta oa mosebetsi o etsoang.
1.3 Boemo ba Biosafety 3 (BSL-3)
BSL-3 e loketse likokoana-hloko tsa matsoalloa kapa tsa kantle ho naha tse nang le monyetla oa phetiso ea aerosol, hammoho le likokoana-hloko tse ka bakang mafu a tebileng le a ka bolaeang.
1.4 Biosafety Level 4 (BSL-4)
BSL-4 e loketse batho ba nang le likokoana-hloko tse tsoang linaheng tse ling tse kotsi le tse sa phekoloeng tse bakang mafu a kotsi ka li-aerosols tse tšoaetsanoang.Liakhente tsena li lekanyelitsoe lilaboratoring tse koaletsoeng haholo.
2. Leqephe la Boitsebiso ba Polokeho (SDS)
Leqephe la lintlha tsa polokeho (SDS), le tsejoang hape e le letlapa la polokeho ea thepa (MSDS), ke foromo e nang le tlhaiso-leseling mabapi le thepa ea lintho tse itseng.SDS e kenyelletsa lintlha tsa 'mele tse kang melting point, beiling point, le flash point, tlhahisoleseding e mabapi le chefo, reactivity, liphello tsa bophelo bo botle, ho boloka le ho lahla ntho, hammoho le lisebelisoa tsa tšireletso tse khothalletsoang le mekhoa ea ho sebetsana le ho lutla.
3. Lisebelisoa tsa Tšireletso
Lisebelisoa tsa polokeho lilaboratoring tsa setso sa lisele li kenyelletsa litšitiso tse kholo, joalo ka likhabinete tsa biosafety, lijana tse koetsoeng, le litsamaiso tse ling tsa boenjiniere tse etselitsoeng ho felisa kapa ho fokotsa ho pepesetsoa lisebelisoa tse kotsi, le lisebelisoa tsa ts'ireletso (PPE) tseo hangata li kopantsoeng le lisebelisoa tse kholo tsa ts'ireletso.Likhabinete tsa polokeho ea baeloji (ke hore li-cell culture hoods) ke lisebelisoa tsa bohlokoa ka ho fetisisa, tse ka laolang ho phatloha ho tšoaetsanoang kapa li-aerosol tse hlahisoang ke mekhoa e mengata ea likokoana-hloko le ho thibela setso sa hau sa lisele hore se se ke sa silafala.
4. Thepa ea Tšireletso ea Motho (PPE)
Thepa ea ts'ireletso ea motho (PPE) ke mokoallo o tobileng pakeng tsa batho le baemeli ba kotsi.Li kenyelletsa lintho tsa tšireletso ea botho, tse kang liatlana, lijase tsa lab le mese, likoahelo tsa lieta, libutsi, li-respirators, lithebe tsa sefahleho, likhalase tsa tšireletso kapa likhalase.Hangata li sebelisoa hammoho le lik'habote tsa polokeho ea baeloji le lisebelisoa tse ling tse nang le li-reagents kapa lisebelisoa tse ntseng li sebetsoa.
Nako ea poso: Feb-01-2023