Cell Culture Media Ke Platform For Customized Development
Mecha ea litaba ea setso sa lisele ke moro oa limatlafatsi o nang le limatlafatsi tsa bohlokoa le lintlha tsa kholo tse hlokahalang bakeng sa kholo le tlhokomelo ea lisele.Hangata e entsoe ka motsoako o leka-lekaneng oa lik'habohaedreite, liprotheine, lipids, liminerale, livithamine le lintlha tsa khōlo.Mecha ea litaba e boetse e fana ka tikoloho e ntle bakeng sa lisele hore li atlehe ho tsona, tse kang pH e nepahetseng, khatello ea osmotic le mocheso.Mecha ea litaba e kanna ea ba le lithibela-mafu ho thibela tšoaetso ea baktheria kapa fungal, le li-additives tse ling ho matlafatsa kholo ea mefuta e itseng ea lisele.Mecha ea litaba ea cell culture e sebelisoa mefuteng e fapaneng ea lipatlisiso le lits'ebetsong tsa bongaka, joalo ka boenjiniere ba lisele, ho sibolla lithethefatsi le lipatlisiso tsa mofetše.
Stem Rekisa Setso Media
Stem cell culture media hangata e na le motsoako oa motsoako oa basal, joalo ka Dulbecco's Modified Eagle Medium (DMEM) kapa RPMI-1640, le serum ea tlatsetso, joalo ka fetal bovine serum (FBS).The basal medium e fana ka limatlafatsi le livithamine tsa bohlokoa, athe serum ea tlatsetso e eketsa lintlha tsa kholo, tse kang insulin, transferrin le selenium.Ho feta moo, li-stem cell culture media media li ka ba le lithibela-mafu, joalo ka penicillin, ho thibela tšoaetso ea libaktheria.Maemong a mang, li-supplement tse ling, joalo ka lintlha tse amanang le kholo, li ka eketsoa mecheng ea litaba tsa setso ho matlafatsa kholo ea lisele tsa stem kapa karohano.
Embryonic Stem Cell ea Motho
Embryonic stem cell (ESCs) ke lisele tsa stem tse nkiloeng ka har'a lisele tse ka hare tsa blastocyst, e leng lesea la pele la ho kenngoa ha lesea.Li-ESC tsa batho li bitsoa li-hESC.Li na le pluripotent, ho bolelang hore li khona ho arola ka mefuta eohle ea lisele tsa likarolo tse tharo tsa mantlha tsa likokoana-hloko: ectoderm, endoderm le mesoderm.Ke sesebelisoa sa bohlokoahali sa ho ithuta baeloji ea nts'etsopele, 'me tšebeliso ea bona e ka bang teng kalafong e nchafatsang ho phekola mafu a mangata e bile sepheo sa lipatlisiso tse ngata.
